Rubidium in PDB 8a35: Nak C-Di Mutant with Rb+ and Na+

The structure of Nak C-Di Mutant with Rb+ and Na+, PDB code: 8a35 was solved by S.Minniberger, A.J.R.Plested, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.03 / 2.05
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	68.062, 68.062, 90.268, 90, 90, 90
R / Rfree (%)	19.2 / 21.7

The structure of Nak C-Di Mutant with Rb+ and Na+ also contains other interesting chemical elements:

Sodium

(Na)

4 atoms

The binding sites of Rubidium atom in the Nak C-Di Mutant with Rb+ and Na+ (pdb code 8a35). This binding sites where shown within 5.0 Angstroms radius around Rubidium atom.
In total 4 binding sites of Rubidium where determined in the Nak C-Di Mutant with Rb+ and Na+, PDB code: 8a35:
Jump to Rubidium binding site number: 1; 2; 3; 4;

Rubidium binding site 1 out of 4 in the Nak C-Di Mutant with Rb+ and Na+


Mono view


Stereo pair view

A full contact list of Rubidium with other atoms in the Rb binding site number 1 of Nak C-Di Mutant with Rb+ and Na+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Rb201 b:69.9 occ:0.25 O A:THR63 2.8 57.0 1.0 O A:VAL64 2.9 69.3 1.0 RB A:RB202 3.5 60.5 0.2 C A:VAL64 3.6 61.5 1.0 C A:THR63 3.9 46.6 1.0 CA A:VAL64 4.2 49.3 1.0 N A:GLY65 4.4 55.0 1.0 N A:VAL64 4.5 45.2 1.0 CA A:GLY65 4.7 58.9 1.0 CA A:THR63 5.0 49.8 1.0

Rubidium binding site 2 out of 4 in the Nak C-Di Mutant with Rb+ and Na+


Mono view


Stereo pair view

A full contact list of Rubidium with other atoms in the Rb binding site number 2 of Nak C-Di Mutant with Rb+ and Na+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Rb202 b:60.5 occ:0.25 O A:THR63 2.8 57.0 1.0 OG1 A:THR63 2.9 48.1 1.0 CB A:THR63 3.3 52.5 1.0 RB A:RB201 3.5 69.9 0.2 C A:THR63 3.7 46.6 1.0 CA A:THR63 4.1 49.8 1.0 CG2 A:THR63 4.5 54.5 1.0 N A:VAL64 4.8 45.2 1.0

Rubidium binding site 3 out of 4 in the Nak C-Di Mutant with Rb+ and Na+


Mono view


Stereo pair view

A full contact list of Rubidium with other atoms in the Rb binding site number 3 of Nak C-Di Mutant with Rb+ and Na+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Rb201 b:58.8 occ:0.25 O B:THR63 2.7 49.5 1.0 OG1 B:THR63 2.8 49.2 1.0 CB B:THR63 3.1 44.8 1.0 C B:THR63 3.6 48.5 1.0 RB B:RB202 3.7 59.6 0.2 CA B:THR63 3.9 43.3 1.0 CG2 B:THR63 4.3 50.5 1.0 N B:VAL64 4.7 50.0 1.0 O B:THR62 5.0 45.4 1.0

Rubidium binding site 4 out of 4 in the Nak C-Di Mutant with Rb+ and Na+


Mono view


Stereo pair view

A full contact list of Rubidium with other atoms in the Rb binding site number 4 of Nak C-Di Mutant with Rb+ and Na+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Rb202 b:59.6 occ:0.25 O B:THR63 2.7 49.5 1.0 O B:VAL64 3.2 59.0 1.0 RB B:RB201 3.7 58.8 0.2 C B:VAL64 3.7 50.0 1.0 C B:THR63 3.8 48.5 1.0 CA B:VAL64 4.1 47.2 1.0 N B:VAL64 4.5 50.0 1.0 N B:GLY65 4.6 46.4 1.0 CA B:THR63 4.9 43.3 1.0 CA B:GLY65 5.0 56.5 1.0

S.Minniberger, S.Abdolvand, S.Braunbeck, H.Sun, A.J.R.Plested. Asymmetry and Ion Selectivity Properties of Bacterial Channel Nak Mutants Derived From Ionotropic Glutamate Receptors. J.Mol.Biol. 67970 2023.
ISSN: ESSN 1089-8638
PubMed: 36682679
DOI: 10.1016/J.JMB.2023.167970